Cornell University Weill Medical College""The Menon Lab
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Publications

Maxfield, F.R., Menon, A.K. (2016)
Intramembrane and Intermembrane Lipid Transport.
Biochemistry of Lipids, Lipoproteins and Membranes (Sixth Edition), edited by N. Ridgway and R.McLeod, Elsevier, pp 415-436.

Hankins H.M., Sere Y.Y., Diab N.S., Menon A.K., Graham T.R. (2015)
Phosphatidylserine translocation at the yeast trans-Golgi network regulates protein sorting into exocytic vesicles.
Mol Biol Cell. 2015 Dec 15;26(25):4674-85.

Menon A.K., Levine T.P. (2015)
Cell biology: Countercurrents in lipid flow.
Nature. 2015 Sep 10;525(7568):191-2.

Verchère A, Menon AK. (2015)
Structural biology: Lipid gymnastics.
Nature. 2015 Aug 27;524(7566):420-2.

Ernst, O.P., Menon, A.K. (2015)
Phospholipid scrambling by rhodopsin.
Photochem. Photobiol. Sci. 2015 Oct 28;14(11):1922-31

Gatta, A.T., Wong, L.H., Sere, Y.Y., Calderón-Noreña, D.M., Cockcroft, S., Menon, A.K., Levine, T.P. (2015)
A new family of StART domain proteins at membrane contact sites has a role in ER-PM sterol transport.
eLife 2015; 10.7554/eLife.07253

Goren, M.A., Morizumi, T., Menon, I., Joseph, J., Dittman, J.S., Cherezov, V., Stevens, R., Ernst, O.P., Menon, A.K. (2014)
Constitutive phospholipid flippase activity of a G protein-coupled receptor.
Nature Commun. 5:5115 doi: 10:1038/ncomms6115 (2014)

Holthuis, J.C.M., Menon, A.K. (2014)
Lipid landscapes and pipelines in membrane homeostasis.

Nature 510, 48-57.

Levine, T.P., Menon, A.K. (2013)
A protein pair with PIPs inside.

Structure 21, 1070-1071.

Malvezzi, M. Chalat, M.N., Janjusevic, R., Picollo, A., Terashima, H., Menon, A.K., Accardi, A. (2013)
Ca2+-dependent phospholipid scrambling by a reconstituted TMEM16 ion channel.

Nature Commun. 4, 2367.

Jelk, J., Gao, N., Serricchio, M., Signorell, A., Schmidt, R.S., Bangs, J.D., Acosta-Serrano, A., Lehrman, M.A., Bütikofer, P., Menon, A.K. (2013)
Glycoprotein biosynthesis in a eukaryote lacking the membrane protein Rft1.

J. Biol. Chem. 288, 20616-20623.

Georgiev, A., Johansen, J., Ramanathan, V.D., Sere, Y.Y., Beh, C.T., Menon, A.K. (2013)
Arv1 regulates PM and ER membrane structure and homeostasis but is dispensable for intracellular sterol transport.

Traffic 14, 912-921.

Beh, C.T., McMaster, C.R., Kozminski, K.G., Menon, A.K. (2012)
A detour for yeast oxysterol-binding protein homologues and nonvesicular trafficking.

J. Biol. Chem. 287, 11481-11488.

Chalat, M., Menon, I., Turan, Z., Menon, A.K. (2012)
Reconstitution of glucosylceramide flip-flop across the endoplasmic reticulum: implications for the mechanism of glycosphingolipid biosynthesis.

J. Biol. Chem. 287, 15523-15532.

Georgiev, A., Sullivan, D.P., Kersting, M.C., Dittman, J.S., Beh, C.T., Menon, A.K. (2011)
Osh proteins regulate membrane sterol organization but are not required for sterol movement between the ER and PM.

Traffic 12, 1341-1355.

Menon, I., Huber, T., Sanyal, S., Banerjee, S., Barré, P., Canis, S., Warren, J.D., Hwa, J., Sakmar, T.P., Menon, A.K. (2011)
Opsin is a phospholipid flippase.

Curr. Biol. 21, 149-153.

Sanyal, S., Menon, A.K. (2010)
Stereoselective transbilayer translocation of mannosyl phosphoryl dolichol by an endoplasmic reticulum flippase.

PNAS 107, 11289-11294.

Sanyal S, Menon AK.
Specific transbilayer translocation of dolichol-linked oligosaccharides by an endoplasmic reticulum flippase.

Proc Natl Acad Sci U S A. 2009 Jan 20;106(3):767-72. Epub 2009 Jan 7.

Beh CT, Alfaro G, Duamel G, Sullivan DP, Kersting MC, Dighe S, Kozminski KG, Menon AK.
Yeast oxysterol-binding proteins: sterol transporters or regulators of cell polarization?
Mol Cell Biochem. 2009 Jan 1. [Epub ahead of print]

Sullivan DP, Georgiev A, Menon AK.
Tritium suicide selection identifies proteins involved in the uptake and intracellular transport of sterols in Saccharomyces cerevisiae.
Eukaryot Cell. 2009 Feb;8(2):161-9. Epub 2008 Dec 5.

Frank CG, Sanyal S, Rush JS, Waechter CJ, Menon AK.
Does Rft1 flip an N-glycan lipid precursor?
Nature. 2008 Jul 31;454(7204):E3-4


Sanyal S, Frank CG, Menon AK.
Distinct flippases translocate glycerophospholipids and oligosaccharide diphosphate dolichols across the endoplasmic reticulum.
Biochemistry. 2008 Jul 29;47(30):7937-46. Epub 2008 Jul 3.

Menon AK.
A flip-flop switch in polarity signaling.
Dev Cell. 2007 Nov;13(5):607-8.

Shukla S, Rai V, Saini P, Banerjee D, Menon AK, Prasad R.
Candida drug resistance protein 1, a major multidrug ATP binding cassette transporter of Candida albicans, translocates fluorescent phospholipids in a reconstituted system.
Biochemistry. 2007 Oct 30;46(43):12081-90. Epub 2007 Oct 9.

Potocky, T., Silvius, J. Menon, A.K., Gellman, S.H.
(2007) HeLa cell entry by guanidinium-rich βbeta-peptides: importance of specific cation-cell surface interactions.
ChemBioChem 8, 917-926

Sutterwala, S.S., Creswell, C.H., Sanyal, S., Menon, A.K., Bangs, J.D. (2007)
De novo sphingolipid synthesis is essential for viability, but not transport of GPI-anchored proteins, in African trypanosomes.
Eukaryotic Cell 6, 454-464

Orlean, P., Menon, A.K.
(2007) GPI anchoring of protein in yeast and mammalian cells or: How we learned to stop worrying and love glycophospholipids.
J. Lipid Res. 48, 993-1011

Vehring, S., Pakkiri, L. Schröer, A., Alder-Baerens, N., Herrmann, A., Menon, A.K., Pomorski, T.
(2007) Flip-flop of fluorescently labeled phospholipids in proteoliposomes reconstituted with yeast microsomal proteins.
Eukaryotic Cell 6, 1625-1634.

Pomorski T, Menon AK.
Lipid flippases and their biological functions.
Cell Mol Life Sci. 2006 Dec;63(24):2908-21.

Vainauskas S, Menon AK.
Ethanolamine phosphate linked to the first mannose residue of glycosylphosphatidylinositol (GPI) lipids is a major feature of the GPI structure that is recognized by human GPI transamidase.
J Biol Chem. 2006 Dec 15;281(50):38358-64.

Maxfield FR, Menon AK.
Intracellular sterol transport and distribution.
Curr Opin Cell Biol. 2006 Aug;18(4):379-85.

Sullivan DP, Ohvo-Rekila H, Baumann NA, Beh CT, Menon AK.
Sterol trafficking between the endoplasmic reticulum and plasma membrane in yeast.
Biochem Soc Trans. 2006 Jun;34(Pt 3):356-8.

Gruszynski AE, van Deursen FJ, Albareda MC, Best A, Chaudhary K,
Cliffe LJ, del Rio L, Dunn JD, Ellis L, Evans KJ, Figueiredo JM,
Malmquist NA, Omosun Y, Palenchar JB, Prickett S, Punkosdy GA, van
Dooren G, Wang Q, Menon AK, Matthews KR, Bangs JD.

Regulation of surface coat exchange by differentiating African trypanosomes.
Mol Biochem Parasitol. 2006 Jun;147(2):211-23.

Vainauskas S, Menon AK.
Endoplasmic reticulum localization of Gaa1 and PIG-T, subunits of the glycosylphosphatidylinositol transamidase complex.
J Biol Chem. 2005 Apr 22;280(16):16402-9.

figureBaumann NA, Sullivan DP, Ohvo-Rekila H, Simonot C, Pottekat A, Klaassen Z, Beh CT, Menon AK.
Transport of newly synthesized sterol to the sterol-enriched plasma membrane occurs via nonvesicular equilibration.
Biochemistry. 2005 Apr 19;44(15):5816-26.

figureVishwakarma RA, Vehring S, Mehta A, Sinha A, Pomorski T, Herrmann A, Menon AK.
New fluorescent probes reveal that flippase-mediated flip-flop of phosphatidylinositol across the endoplasmic reticulum membrane does not depend on the stereochemistry of the lipid.
Org Biomol Chem. 2005 Apr 7;3(7):1275-83.

Potocky TB, Menon AK, Gellman SH.
Effects of conformational stability and geometry of guanidinium display on cell entry by beta-peptides.
J Am Chem Soc. 2005 Mar 23;127(11):3686-7.

Vishwakarma RA, Menon AK.
Flip-flop of glycosylphosphatidylinositols (GPI's) across the ER.
Chem Commun (Camb). 2005 Jan 28;(4):453-5.

Chang QL, Gummadi SN, Menon AK.
Chemical modification identifies two populations of glycerophospholipid flippase in rat liver ER.
Biochemistry. 2004 Aug 24;43(33):10710-8.

Pottekat A, Menon AK.
Subcellular localization and targeting of N-acetylglucosaminyl phosphatidylinositol de-N-acetylase, the second enzyme in the glycosylphosphatidylinositol biosynthetic pathway.
J Biol Chem. 2004 Apr 16;279(16):15743-51.

figureVainauskas S, Menon AK.
A conserved proline in the last transmembrane segment of Gaa1 is required for glycosylphosphatidylinositol (GPI) recognition by GPI transamidase.
J Biol Chem. 2004 Feb 20;279(8):6540-5.

Potocky TB, Menon AK, Gellman SH.
Cytoplasmic and nuclear delivery of a TAT-derived peptide and a beta-peptide after endocytic uptake into HeLa cells.
J Biol Chem. 2003 Dec 12;278(50):50188-94.

Kostova Z, Yan BC, Vainauskas S, Schwartz R, Menon AK, Orlean P.
Comparative importance in vivo of conserved glutamate residues in the EX7E motif retaining glycosyltransferase Gpi3p, the UDP-GlcNAc-binding subunit of the first enzyme in glycosylphosphatidylinositol assembly.
Eur J Biochem. 2003 Nov;270(22):4507-14.

Gummadi SN, Hrafnsdottir S, Walent J, Watkins WE, Menon AK.
Reconstitution and assay of biogenic membrane-derived phospholipid flippase activity in proteoliposomes.
In "Membrane Protein Protocols: Expression, Purification, and Crystallization”, B.S. Selinsky (ed.), Humana Press series ‘Methods in Molecular Biology’, 228: 271-279

figureWatkins WE, Menon AK.
Reconstitution of phospholipid flippase activity from E. coli inner membrane: a test of the protein translocon as a candidate flippase.
Biol Chem. 2002 Sep;383(9):1435-40.


Vainauskas S, Maeda Y, Kurniawan H, Kinoshita T, Menon AK.
Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex.
J Biol Chem. 2002 Aug 23;277(34):30535-42.

Gummadi SN, Menon AK.
Transbilayer movement of dipalmitoylphosphatidylcholine in proteoliposomes reconstituted from detergent extracts of endoplasmic reticulum. Kinetics of transbilayer transport mediated by a single flippase and identification of protein fractions enriched in flippase activity.
J Biol Chem. 2002 Jul 12;277(28):25337-43.

coverMenon AK.
Introduction: lipid transport--an overview.
Semin Cell Dev Biol. 2002 Jun;13(3):159-62.







Kubelt J, Menon AK, Muller P, Herrmann A.
Transbilayer movement of fluorescent phospholipid analogues in the cytoplasmic membrane of Escherichia coli.
Biochemistry. 2002 Apr 30;41(17):5605-12.

Vidugiriene J, Vainauskas S, Johnson AE, Menon AK.
Endoplasmic reticulum proteins involved in glycosylphosphatidylinositol-anchor attachment: photocrosslinking studies in a cell-free system.
Eur J Biochem. 2001 Apr;268(8):2290-300.

Li J, Rancour DM, Allende ML, Worth CA, Darling DS, Gilbert JB, Menon AK, Young WW Jr.
The DXD motif is required for GM2 synthase activity but is not critical for nucleotide binding.
Glycobiology. 2001 Mar;11(3):217-29.

Sharma DK, Hilley JD, Bangs JD, Coombs GH, Mottram JC, Menon AK.
Soluble GPI8 restores glycosylphosphatidylinositol anchoring in a trypanosome cell-free system depleted of lumenal endoplasmic reticulum proteins.
Biochem J. 2000 Nov 1;351 Pt 3:717-22.

Kostova Z, Rancour DM, Menon AK, Orlean P.
Photoaffinity labelling with P3-(4-azidoanilido)uridine 5'-triphosphate identifies gpi3p as the UDP-GlcNAc-binding subunit of the enzyme that catalyses formation of GlcNAc-phosphatidylinositol, the first glycolipid intermediate in glycosylphosphatidylinositol synthesis.
Biochem J. 2000 Sep 15;350 Pt 3:815-22.

figureHrafnsdottir S, Menon AK.
Reconstitution and partial characterization of phospholipid flippase activity from detergent extracts of the Bacillus subtilis cell membrane.
J Bacteriol. 2000 Aug;182(15):4198-206.

Baumann NA, Vidugiriene J, Machamer CE, Menon AK.
Cell surface display and intracellular trafficking of free glycosylphosphatidylinositols in mammalian cells.
J Biol Chem. 2000 Mar 10;275(10):7378-89.

Menon AK, Watkins WE3rd, Hrafnsdottir S.
Specific proteins are required to translocate phosphatidylcholine bidirectionally across the endoplasmic reticulum.
Curr Biol. 2000 Mar 9;10(5):241-52.

McConville MJ, Menon AK.
Recent developments in the cell biology and biochemistry of glycosylphosphatidylinositol lipids (review).
Mol Membr Biol. 2000 Jan-Mar;17(1):1-16. Review.

Sharma DK, Vidugiriene J, Bangs JD, Menon AK.
A cell-free assay for glycosylphosphatidylinositol anchoring in African trypanosomes. Demonstration of a transamidation reaction mechanism.
J Biol Chem. 1999 Jun 4;274(23):16479-86.

Vidugiriene J, Sharma DK, Smith TK, Baumann NA, Menon AK.
Segregation of glycosylphosphatidylinositol biosynthetic reactions in a subcompartment of the endoplasmic reticulum.
J Biol Chem. 1999 May 21;274(21):15203-12.

Puglielli L, Mandon EC, Rancour DM, Menon AK, Hirschberg CB.
Identification and purification of the rat liver Golgi membrane UDP-N-acetylgalactosamine transporter.
J Biol Chem. 1999 Feb 12;274(7):4474-9.

Menon AK.
Lipids: more than just membrane fabric.
Trends Cell Biol. 1998 Sep;8(9):374-6. No abstract available.

Rancour DM, Menon AK.
Identification of endoplasmic reticulum proteins involved in glycan assembly: synthesis and characterization of P3-(4-azidoanilido)uridine 5'-triphosphate, a membrane-topological photoaffinity probe for uridine diphosphate-sugar binding proteins.
Biochem J. 1998 Aug 1;333 ( Pt 3):661-9.

Menon AK, Baumann NA, van't Hof W, Vidugiriene J.
Glycosylphosphatidylinositols: biosynthesis and intracellular transport.
Biochem Soc Trans. 1997 Aug;25(3):861-5. Review. No abstract available.

Hrafnsdottir S, Nichols JW, Menon AK.
Transbilayer movement of fluorescent phospholipids in Bacillus megaterium membrane vesicles.
Biochemistry. 1997 Apr 22;36(16):4969-78.

Butikofer P, Boschung M, Brodbeck U, Menon AK.
Phosphatidylinositol hydrolysis by Trypanosoma brucei glycosylphosphatidylinositol phospholipase C.
J Biol Chem. 1996 Jun 28;271(26):15533-41.

van't Hof W, Rodriguez-Boulan E, Menon AK.
Nonpolarized distribution of glycosylphosphatidylinositols in the plasma membrane of polarized Madin-Darby canine kidney cells.
J Biol Chem. 1995 Oct 13;270(41):24150-5.

Vidugiriene J, Menon AK.
Soluble constituents of the ER lumen are required for GPI anchoring of a model protein.
EMBO J. 1995 Oct 2;14(19):4686-94.

Menon AK.
Flippases.
Trends Cell Biol. 1995 Sep;5(9):355-60.

Butikofer P, Boschung M, Menon AK.
Production of a nested set of glycosylphosphatidylinositol structures from a glycosylphosphatidylinositol-anchored protein.
Anal Biochem. 1995 Jul 20;229(1):125-32.

Vidugiriene J, Menon AK.
Biosynthesis of glycosylphosphatidylinositol anchors.
Methods Enzymol. 1995;250:513-35. No abstract available.

Vidugiriene J, Menon AK.
The GPI anchor of cell-surface proteins is synthesized on the cytoplasmic face of the endoplasmic reticulum.
J Cell Biol. 1994 Oct;127(2):333-41.

Menon AK, Vidugiriene J.
Topology of GPI biosynthesis in the endoplasmic reticulum.
Braz J Med Biol Res. 1994 Feb;27(2):167-75.

Menon AK.
Structural analysis of glycosylphosphatidylinositol anchors.
Methods Enzymol. 1994;230:418-42.

Vidugiriene J, Menon AK.
Early lipid intermediates in glycosyl-phosphatidylinositol anchor assembly are synthesized in the ER and located in the cytoplasmic leaflet of the ER membrane bilayer.
J Cell Biol. 1993 Jun;121(5):987-96.

Menon AK, Eppinger M, Mayor S, Schwarz RT.
Phosphatidylethanolamine is the donor of the terminal phosphoethanolamine group in trypanosome glycosylphosphatidylinositols.
EMBO J. 1993 May;12(5):1907-14.

Patnaik PK, Field MC, Menon AK, Cross GA, Yee MC, Butikofer P.
Molecular species analysis of phospholipids from Trypanosoma brucei bloodstream and procyclic forms.
Mol Biochem Parasitol. 1993 Mar;58(1):97-105.

Kamitani T, Menon AK, Hallaq Y, Warren CD, Yeh ET.
Complexity of ethanolamine phosphate addition in the biosynthesis of glycosylphosphatidylinositol anchors in mammalian cells.
J Biol Chem. 1992 Dec 5;267(34):24611-9.

Menon AK, Stevens VL.
Phosphatidylethanolamine is the donor of the ethanolamine residue linking a glycosylphosphatidylinositol anchor to protein.
J Biol Chem. 1992 Aug 5;267(22):15277-80.

Field MC, Menon AK, Cross GA.
Developmental variation of glycosylphosphatidylinositol membrane anchors in Trypanosoma brucei. In vitro biosynthesis of intermediates in the construction of the GPI anchor of the major procyclic surface glycoprotein.
J Biol Chem. 1992 Mar 15;267(8):5324-9.

Mayor S, Menon AK, Cross GA.
Galactose-containing glycosylphosphatidylinositols in Trypanosoma brucei.
J Biol Chem. 1992 Jan 15;267(2):754-61.

Menon AK.
Biosynthesis of glycosyl-phosphatidylinositol.
Cell Biol Int Rep. 1991 Nov;15(11):1007-21. Review. No abstract available.

Low P, Dallner G, Mayor S, Cohen S, Chait BT, Menon AK.
The mevalonate pathway in the bloodstream form of Trypanosoma brucei. Identification of dolichols containing 11 and 12 isoprene residues.
J Biol Chem. 1991 Oct 15;266(29):19250-7.

Field MC, Menon AK, Cross GA.
A glycosylphosphatidylinositol protein anchor from procyclic stage Trypanosoma brucei: lipid structure and biosynthesis.
EMBO J. 1991 Oct;10(10):2731-9.

Lisanti MP, Field MC, Caras IW, Menon AK, Rodriguez-Boulan E.
Mannosamine, a novel inhibitor of glycosylphosphatidylinositol incorporation into proteins.
EMBO J. 1991 Aug;10(8):1969-77.

Mayor S, Menon AK, Cross GA.
Transfer of glycosyl-phosphatidylinositol membrane anchors to polypeptide acceptors in a cell-free system.
J Cell Biol. 1991 Jul;114(1):61-71.

Field MC, Menon AK, Cross GA.
Developmental variation of glycosylphosphatidylinositol membrane anchors in Trypanosoma brucei. Identification of a candidate biosynthetic precursor of the glycosylphosphatidylinositol anchor of the major procyclic stage surface glycoprotein.
J Biol Chem. 1991 May 5;266(13):8392-400.

Menon AK, Mayor S, Schwarz RT.
Biosynthesis of glycosyl-phosphatidylinositol lipids in Trypanosoma brucei: involvement of mannosyl-phosphoryldolichol as the mannose donor.
EMBO J. 1990 Dec;9(13):4249-58.

Menon AK, Schwarz RT, Mayor S, Cross GA.
Cell-free synthesis of glycosyl-phosphatidylinositol precursors for the glycolipid membrane anchor of Trypanosoma brucei variant surface glycoproteins. Structural characterization of putative biosynthetic intermediates.
J Biol Chem. 1990 Jun 5;265(16):9033-42.

Mayor S, Menon AK, Cross GA.
Glycolipid precursors for the membrane anchor of Trypanosoma brucei variant surface glycoproteins. II. Lipid structures of phosphatidylinositol-specific phospholipase C sensitive and resistant glycolipids.
J Biol Chem. 1990 Apr 15;265(11):6174-81.

Mayor S, Menon AK, Cross GA, Ferguson MA, Dwek RA, Rademacher TW.
Glycolipid precursors for the membrane anchor of Trypanosoma brucei variant surface glycoproteins. I. Can structure of the phosphatidylinositol-specific phospholipase C sensitive and resistant glycolipids.
J Biol Chem. 1990 Apr 15;265(11):6164-73.

Menon AK, Mayor S, Ferguson MA, Duszenko M, Cross GA.
Candidate glycophospholipid precursor for the glycosylphosphatidylinositol membrane anchor of Trypanosoma brucei variant surface glycoproteins.
J Biol Chem. 1988 Feb 5;263(4):1970-7.

Menon AK, Holowka D, Webb WW, Baird B.
Cross-linking of receptor-bound IgE to aggregates larger than dimers leads to rapid immobilization.
J Cell Biol. 1986 Feb;102(2):541-50.

Menon AK, Holowka D, Webb WW, Baird B.
Clustering, mobility, and triggering activity of small oligomers of immunoglobulin E on rat basophilic leukemia cells.
J Cell Biol. 1986 Feb;102(2):534-40.

Menon AK, Holowka D, Baird B.
Small oligomers of immunoglobulin E (IgE) cause large-scale clustering of IgE receptors on the surface of rat basophilic leukemia cells.
J Cell Biol. 1984 Feb;98(2):577-83.

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